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| 产品名称： | B27M2 |
|---|---|
| 商品货号： | TS212631 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Animals were immunized with human peripheral blood leukocytes (HLA phenotype A2,A28,B27,B44,Bw4,Cw2). Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against HLA B27, Bw47; cytotoxic |
| Cellular Products： | immunoglobulin; monoclonal antibody; against HLA B27, Bw47; cytotoxic |
| Comments： | Animals were immunized with human peripheral blood leukocytes (HLA phenotype A2,A28,B27,B44,Bw4,Cw2). Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells. The antibody is cytotoxic for a subpopulation of HLA B27 positive cells and for all HLA Bw47 positive cells at an appropriate titer. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Culture Medium: Modified RPMI 1640 medium (see below) with 50 uM 2-mercaptoethanol, 90%; fetal bovine serum, 10%. RPMI 1640 100 mL L-Glutamine (100x) 1 mL Solution FM 1 mL Non-essential amino acids (100x) 1 mL Fetal Bovine Serum 12 mL Solution FM (100x) 1. 1320 mg oxalacetic acid (100 mM, MW 132). 2. 100 mg crystalline bovine insulin (20 units/mL, 25 units/mg). Add 1 and 2, stir at 37°C. Add Na pyruvate 450 mg (41 mM, FW 110). Bring up to 100 mL with distilled water. Stir at 37°C until solution dissolves. Filter, aliquot and store frozen. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgM |
| Name of Depositor： | F Grumet |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Grumet FC, et al. Monoclonal antibody (B27M2) subdividing HLA-B27. Hum. Immunol. 5: 61-72, 1982. PubMed: 6981637 |