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微信小章| 产品名称： | B1B3 464 B1B3 |
|---|---|
| 商品货号： | TS212640 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody binds to an epitope in amino acids 3506 through 3635 on human apolipoprotein B. |
| Derivation： | Animals were immunized with human low density lipoprotein (LDL) obtained from plasma from a donor with familial hypercholesterolemia. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Clinical Data： | Animals were immunized with human low density lipoprotein (LDL) obtained from plasma from a donor with familial hypercholesterolemia. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human low density lipoprotein |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human low density lipoprotein |
| Tumorigenic： | Yes |
| Effects： | Yes, forms ascites in pristane primed BALB/c mice |
| Comments： | Animals were immunized with human low density lipoprotein (LDL) obtained from plasma from a donor with familial hypercholesterolemia. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody binds to an epitope in amino acids 3506 through 3635 on human apolipoprotein B. It cross competes with antibody B1B6 (see ATCC CRL-2248). The antibody blocks binding of LDL to LDL receptors. It does not bind apolipoprotein B48. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose, 0.1 mM non-essential amino acids and 1 mM sodium pyruvate, 85%; horse serum, 10%; fetal bovine serum, 5%
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| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Isotype： | IgG1 |
| Name of Depositor： | G Schonfeld |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Krul ES, et al. Roles of apolipoproteins B and E in the cellular binding of very low density lipoproteins. J. Clin. Invest. 75: 361-369, 1985. PubMed: 3973009 Hahm KS, et al. Limited proteolysis selectively destroys epitopes on apolipoprotein B in low density lipoproteins. J. Lipid Res. 24: 877-885, 1983. PubMed: 6195279 Krul ES, et al. Regional specificities of monoclonal anti-human apolipoprotein B antibodies. J. Lipid Res. 29: 937-947, 1988. PubMed: 2457642 Kinoshita M, et al. Modification of the core lipids of low density lipoproteins produces selective alterations in the expression of apoB-100 epitopes. J. Lipid Res. 31: 701-708, 1990. PubMed: 1693649 Tikkanen MJ, et al. Expression of apolipoprotein B epitopes in very low density lipoprotein subfractions. Studies with monoclonal antibodies. Arteriosclerosis 4: 138-146, 1984. PubMed: 6200098 Krul ES, et al. Two distinct truncated apolipoprotein B species in a kindred with hypobetalipoproteinemia. Arteriosclerosis 9: 856-868, 1989. PubMed: 2574033 Tikkanen NJ, et al. Antigenic mapping of human low density lipoprotein with monoclonal antibodies. J. Lipid Res. 23: 1032-1038, 1982. PubMed: 6183385 Tikkanen MJ, et al. Differential reactivity of human low density lipoproteins with monoclonal antibodies. J. Lipid Res. 24: 1494-1499, 1983. PubMed: 6197500 Krul ES, et al. Heterogeneity of apolipoprotein E epitope expression on human lipoproteins: importance for apolipoprotein E function. J. Lipid Res. 29: 1309-1325, 1988. PubMed: 2466929 |