微信小陈
微信小章| 产品名称： | B13-24 |
|---|---|
| 商品货号： | TS212643 |
| Organism： | Cricetulus griseus, hamster, Chinese |
| Tissue： | ovary |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 2 CELLS CONTAIN SV-40-CMV VIRAL DNA SEQUENCES
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | female |
| Applications： | The cell line was derived by co-transfecting the CHO cell line DXB11 using Lipofection with heavy and light chain expression plasmids constructed from the antibody produced by the 23F2G cell line (ATCC-HB-11081). The cell line is capable of producing about 100 micrograms/liter of the humanized immunoglobulin at the rate of 25 pg/cell/day. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | The cell line was derived by co-transfecting the CHO cell line DXB11 using Lipofection with heavy and light chain expression plasmids constructed from the antibody produced by the 23F2G cell line (ATCC-HB-11081). |
| Clinical Data： | female |
| Comments： | This line is Neomycin resistant.xa0 The cell line was derived by co-transfecting the CHO cell line DXB11 using Lipofection with heavy and light chain expression plasmids constructed from the antibody produced by the 23F2G cell line (ATCC-HB-11081).xa0 The plasmids contain both cytomegalovirus (CMV) and SV40 viral sequences.xa0 The 23F2G antibody is reactive with the beta chain (CD18) of human leukocyte integrins. The cell line is capable of producing about 100 micrograms/liter of the humanized immunoglobulin at the rate of 25 pg/cell/day. |
| Complete Growth Medium： | Iscoves modified Dulbeccos medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 90%; heat-inactivated fetal bovine serum, 10%
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:4 to 1:6 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG4 |
| Name of Depositor： | ICOS Corporation |
| Deposited As： | hamster, Chinese |
| U.S. Patent Number： | |
| References： | Rose LM. Murine and humanizer 23F2G antibodies and cell lines expressing said antibodies. US Patent 5,854,070 dated Dec 29 1998 Bowen JD, et al. Phase I study of a humanized anti-CD11/CD18 monoclonal antibody in multiple sclerosis. Clin. Pharmacol. Ther. 64: 339-346, 1998. PubMed: 9757158 Yenari MA, et al. Hu23F2G, an antibody recognizing the leukocyte CD11/CD18 integrin, reduces injury in a rabbit model of transient focal cerebral ischemia. Exp. Neurol. 153: 223-233, 1998. PubMed: 9784282 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |