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| 产品名称： | B27M1 |
|---|---|
| 商品货号： | TS212647 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Animals were immunized with peripheral blood leukocytes from a normal human female (HLA phenotype A2,A25,B8,B27,Cw1,Cw7). Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with Sp2/08A2 myeloma cells. |
| Clinical Data： | Animals were immunized with peripheral blood leukocytes from a normal human female (HLA phenotype A2,A25,B8,B27,Cw1,Cw7). |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against HLA B27, B7; cytotoxic |
| Cellular Products： | immunoglobulin; monoclonal antibody; against HLA B27, B7; cytotoxic |
| Comments： | Animals were immunized with peripheral blood leukocytes from a normal human female (HLA phenotype A2,A25,B8,B27,Cw1,Cw7). Spleen cells were fused with Sp2/08A2 myeloma cells. The antibody is cytotoxic for HLA B27 and for HLA B7 at appropriate dilution. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 5 X 10 exp5 cells/ml. |
| Isotype： | IgG2a |
| Name of Depositor： | F Grumet |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Grumet FC, et al. Monoclonal anti-HLA-B27 antibody (B27M1): production and lack of detectable typing difference between patients with ankylosing spondylitis, Reiters syndrome, and normal controls. Lancet 2: 174-176, 1981. PubMed: 6166821 |