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微信小章| 产品名称： | AtT-20/D16v-F2 |
|---|---|
| 商品货号： | TS212648 |
| Organism： | Mus musculus, mouse |
| Tissue： | pituitary, anterior |
| Product Format： | frozen |
| Morphology： | small rounded cells |
| Culture Properties： | loosely adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | tumor |
| Strain： | LAF1 |
| Applications： | This clone has been used successfully by Moore et al. for several DNA mediated transfection studies relating to endocrine and exocrine secretory pathways.
The cells are readily transfected using a standard calcium phosphate protocol. |
| Storage Conditions： | liquid nitrogen vaor phase |
| Images： |  |
| Genes Expressed： | adrenocorticotropic hormone (ACTH) |
| Cellular Products： | adrenocorticotropic hormone (ACTH) |
| Comments： | The F2 subclone of AtT-20 (see ATCC CCL-89) was developed by B. Gumbiner.
Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cells grow in patches and pile up. Cultures do not become confluent.
Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: Three times weekly |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vaor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor： | RB Kelly |
| Deposited As： | Mus musculus |
| References： | Gumbiner B, Kelly RB. Two distinct intracellular pathways transport secretory and membrane glycoproteins to the surface of pituitary tumor cells. Cell 28: 51-59, 1982. PubMed: 6279313 Moore HP, et al. Expressing a human proinsulin cDNA in a mouse ACTH-secreting cell. Intracellular storage, proteolytic processing, and secretion on stimulation. Cell 35: 531-538, 1983. PubMed: 6317196 Burgess TL, et al. The exocrine protein trypsinogen is targeted into the secretory granules of an endocrine cell line: studies by gene transfer. J. Cell Biol. 101: 639-645, 1985. PubMed: 2991303 Buonassisi V, et al. Hormone-producing cultures of adrenal and pituitary tumor origin. Proc. Natl. Acad. Sci. USA 48: 1184-1190, 1962. PubMed: 13874682 Stefana B, et al. Leukemia inhibitory factor induces differentiation of pituitary corticotroph function: an immuno-neuroendocrine phenotypic switch. Proc. Natl. Acad. Sci. USA 93: 12502-12506, 1996. PubMed: 8901611 |
| Cross References： | Nucleotide (GenBank) : U79773 Mus musculus NNP-1 (NNP-1) mRNA, complete cds. Nucleotide (GenBank) : U79774 Mus musculus NNP-1 var (NNP-1) mRNA, complete cds. Nucleotide (GenBank) : NM_010925 Mus musculus novel nuclear protein 1 (Nnp1), mRNA. |