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微信小章| 产品名称： | As4.1 |
|---|---|
| 商品货号： | TS212650 |
| Organism： | Mus musculus, mouse |
| Tissue： | kidney |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 xa0Cells contain papovavirus
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | female |
| Applications： | transfection host |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | As4.1 was established from the ascites fluid of a six month old transgenic mouse harboring an intraparenchymal kidney tumor induced by transgene targeted tumorigenesis. These transgenic mice were constructed with the renin T antigen fusion gene, pR2d4.6TAG which contains a transcriptional fusion between Ren-2d 5 flanking sequences and the structural gene for SV40 T antigen. |
| Clinical Data： | female |
| Genes Expressed： | renin |
| Cellular Products： | renin |
| Comments： | The cell line was cloned by picking a small cluster from a dish seeded at low density.
The As4.1 cells express high levels of correctly processed renin mRNA from the endogenous Ren-1c locus.
They constitutively secrete prorenin and appear to store a proteolytically cleaved form of active renin intracellularly in storage granules/vesicles.
It has been reported that each As4.1 cell contains 1000 to 2000 copies of renin mRNA. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:6 to 1:12 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | KW Gross |
| Deposited As： | Mus musculus |
| References： | Jones CA, et al. Expression of murine renin genes during fetal development. Mol. Endocrinol. 4: 375-383, 1990. PubMed: 2188116 Sigmund CD, et al. Tissue and cell specific expression of a renin promoter-reporter gene construct in transgenic mice. Biochem. Biophys. Res. Commun. 170: 344-350, 1990. PubMed: 1695507 Sigmund CD, et al. Isolation and characterization of renin-expressing cell lines from transgenic mice containing a renin-promoter viral oncogene fusion construct. J. Biol. Chem. 265: 19916-19922, 1990. PubMed: 2174057 Petrovic N, et al. Role of proximal promoter elements in regulation of renin gene transcription. J. Biol. Chem. 271: 22499-22505, 1996. PubMed: 8798416 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |