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微信小章| 产品名称： | AsPC-1 |
|---|---|
| 商品货号： | TS212651 |
| Organism： | Homo sapiens, human |
| Tissue： | pancreas; derived from metastatic site: ascites |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenocarcinoma |
| Age： | 62 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was derived from nude mouse xenografts initiated with cells from the ascites of a patient with cancer of the pancreas. |
| Clinical Data： | 62 years
Caucasian
female
|
| Genes Expressed： | carcinoembryonic antigen (CEA); human pancreas associated antigen; human pancreas specific antigen; mucin |
| Cellular Products： | carcinoembryonic antigen (CEA); human pancreas associated antigen; human pancreas specific antigen; mucin |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.xa0Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,13 D13S317: 9,12 D16S539: 11 D5S818: 12 D7S820: 12,13 THO1: 7,9.3 TPOX: 8,10 vWA: 17 |
| Name of Depositor： | MH Tan |
| Deposited As： | Homo sapiens |
| References： | Chen WH, et al. Human pancreatic adenocarcinoma: in vitro and in vivo morphology of a new tumor line established from ascites. In Vitro 18: 24-34, 1982. PubMed: 7182348 Tan MH, et al. Differential localization of human pancreas cancer-associated antigen and carcinoembryonic antigen in homologous pancreatic tumoral xenograft. J. Natl. Cancer Inst. 67: 563-569, 1981. PubMed: 6944528 Loor R, et al. Use of pancreas-specific antigen in immunodiagnosis of pancreatic cancer. Clin. Lab. Med. 2: 567-578, 1982. PubMed: 6754226 Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903 Tan MH, Chu TM. Characterization of the tumorigenic and metastatic properties of a human pancreatic tumor cell line (AsPC-1) implanted orthotopically into nude mice. Tumor Biol. 6: 89-98, 1985. PubMed: 4023565 |
| Cross References： | Nucleotide (GenBank) : AA640215 01 subtracted 3 EST library Homo sapiens cDNA clone U32, mRNA sequence. Nucleotide (GenBank) : AA640216 02 subtracted 3 EST library Homo sapiens cDNA clone U30, mRNA sequence. Nucleotide (GenBank) : AA640217 03 subtracted 3 EST library Homo sapiens cDNA clone D32, mRNA sequence. Nucleotide (GenBank) : AA640218 04 subtracted 3 EST library Homo sapiens cDNA clone D42, mRNA sequence. |