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微信小章| 产品名称： | AtT-20 |
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| 商品货号： | TS212652 |
| Organism： | Mus musculus, mouse |
| Tissue： | pituitary |
| Product Format： | frozen |
| Morphology： | small rounded cells |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | tumor |
| Strain： | LAF1 |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | Clone AtT-20, an ACTH secreting cell line, was cloned in October, 1966, by G. Sato and associates from earlier cultures established after alternate passage of mouse pituitary tumor cells as tumors in animals and in cell culture. (The cells were cultured in Hams F10 medium, 82.5%; horse serum, 15%; FBS, 2.5%). The mouse pituitary tumor was originally established in LAF1 mice by J. Furth, et al. This clone has now been carried in culture without alternate animal passage. |
| Genes Expressed： | adrenocorticotropic hormone (ACTH) |
| Cellular Products： | adrenocorticotropic hormone (ACTH) |
| Virus Susceptibility： | Vesicular stomatitis virus
Human poliovirus 1 |
| Comments： | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.
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| Subculturing： | Allow clusters to settle and remove all but 5 to 10 mL of medium. Gently transfer the clusters to new flasks and add fresh medium. Proliferation is enhanced by incubation on a shaking platform.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Note: The cells do not attach but aggregate to form clusters; the clusters are viable. |
| Cryopreservation： | Freeze medium: culture medium, 90%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor： | G Sato |
| Deposited As： | Mus musculus |
| Passage History： | This clone has now been carried in culture without alternate animal passage. |
| Year of Origin： | October, 1966 |
| References： | Buonassisi V, et al. Hormone-producing cultures of adrenal and pituitary tumor origin. Proc. Natl. Acad. Sci. USA 48: 1184-1190, 1962. PubMed: 13874682 Furth J, et al. ACTH secreting transplantable pituitary tumors. Proc. Soc. Exp. Biol. Med. 84: 253-254, 1953. PubMed: 13121002 Tabernero C, et al. Identification of an RNA sequence within an intracisternal-A particle element able to replace Rev-mediated posttranscriptional regulation of human immunodeficiency virus type 1. J. Virol. 71: 95-101, 1997. PubMed: 8985327 Gamby C, et al. Growth-associated protein-43 (GAP-43) facilitates peptide hormone secretion in mouse anterior pituitary AtT-20 cells. J. Biol. Chem. 271: 10023-10028, 1996. PubMed: 8626556 |