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微信小章| 产品名称： | B104-1-1 |
|---|---|
| 商品货号： | TS212657 |
| Organism： | Mus musculus, mouse |
| Tissue： | embryo |
| Cell Type： | neuroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Neuroblastoma, Glioblastoma |
| Age： | embryo |
| Strain： | NIH/Swiss |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line was established by A.L. Schechter et al. in 1984 by transfecting NIH/3T3 cells with EcoR1 digested DNA from the rat neuroblastoma cell line B-104. |
| Comments： | The cells contain the neu transforming gene which codes for a 185000 dalton antigen designated p185. The p185 protein is strongly associated with the presence of glioblastoma and neuroblastoma oncogenes. The neu oncogene is homologous to the erb-B oncogene, and p185 is serologically similar to the epidermal growth factor receptor. |
| Complete Growth Medium： | ATCC formulated DMEM (ATCC®xa030-2002) supplemented with 10% bovine calf serumxa0(ATCC®xa030-2030) |
| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:100 is recommended Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.xa0xa0 xa0 |
| Name of Depositor： | RA Weinberg |
| Deposited As： | Mus musculus |
| Year of Origin： | 1984 |
| References： | Schechter AL, et al. The neu oncogene: an erb-B-related gene encoding a 185,000-Mr tumour antigen. Nature 312: 513-516, 1984. PubMed: 6095109 |