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微信小章| 产品名称： | ARIP |
|---|---|
| 商品货号： | TS212662 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | pancreas |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | pancreatic tumor |
| Strain： | Wistar |
| Storage Conditions： | liquid nitrogen vapor phase |
| Genes Expressed： | exocrine enzymes (low levels) |
| Cellular Products： | exocrine enzymes (low levels) |
| Tumorigenic： | No |
| Effects： | Yes, did form colonies in semisolid medium. No, the cells were not tumorigenic in immunosuppressed mice |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:3 to 1:6 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | NW Jessop, RJ Hay |
| Deposited As： | Rattus sp. |
| References： | Jessop NW, Hay RJ. Characteristics of two rat pancreatic exocrine cell lines derived from transplantable tumors. In Vitro 16: 212, 1980. Cockell M, et al. Identification of a cell-specific DNA-binding activity that interacts with a transcriptional activator of genes expressed in the acinar pancreas. Mol. Cell. Biol. 9: 2464-2476, 1989. PubMed: 2788241 Roux E, et al. The cell-specific transcription factor PTF1 contains two different subunits that interact with the DNA. Genes Dev. 3: 1613-1624, 1989. PubMed: 2612907 Hui H, et al. Glucagon-like peptide 1 induces differentiation of islet duodenal homeobox-1-positive pancreatic ductal cells into insulin-secreting cells. Diabetes 50: 785-796, 2001. PubMed: 11289043 Silver K, Yao F. ARIP cells as a model for pancreatic beta cell growth and development. Pancreas 22: 141-147, 2001. PubMed: 11249068 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |