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微信小章| 产品名称： | ARPE-19/HPV-16 |
|---|---|
| 商品货号： | TS212666 |
| Organism： | Homo sapiens, human |
| Tissue： | eye; retinal pigmented epithelium |
| Cell Type： | human papillomavirus 16 (HPV-16) transfected |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain Human Papilloma viral 16 (HPV16) sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 19 years |
| Gender： | male |
| Applications： | transfection host |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | diploid |
| Derivation： | The ARPE/HPV-16 transformed cell line was derived from the ARPE-19 cell line (ATCC CRL-2302) by transfection with DH5-HPV-16. ARPE-19 is a spontaneously arising retinal pigment epithelia (RPE) cell line derived in 1986 by Amy Aotaki-Keen from the normal eyes of a 19-year-old male who died from head trauma in a motor vehicle accident. |
| Clinical Data： | ARPE-19 is a spontaneously arising retinal pigment epithelia (RPE) cell line derived in 1986 by Amy Aotaki-Keen from the normal eyes of a 19-year-old male who died from head trauma in a motor vehicle accident. male |
| Antigen Expression： | RPE-specific markers CRALBP and RPE-65 Ref   Dunn KC, et al. ARPE-19, A human retinal pigment epithelial cell line with differentiated properties. Exp. Eye Res. 62: 155-169, 1996. PubMed: 8698076 |
| Genes Expressed： | RPE-specific markers CRALBP and RPE-65 |
| Comments： | The ARPE/HPV-16 transformed cell line was derived from the ARPE-19 cell line (ATCC CRL-2302) by transfection with DH5-HPV-16. ARPE-19 is a spontaneously arising retinal pigment epithelia (RPE) cell line derived in 1986 by Amy Aotaki-Keen from the normal eyes of a 19-year-old male who died from head trauma in a motor vehicle accident. The cells express the RPE-specific markers CRALBP and RPE-65. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:4 to 1:8 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney,xa05th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X, Y CSF1PO: 11 D13S317: 11, 12 D16S539: 9, 11 D5S818: 13 D7S820: 9, 11 THO1: 6, 9.3 TPOX: 9, 11 vWA: 16, 19 |
| Name of Depositor： | JW Obringer |
| Deposited As： | human |
| References： | Dunn KC, et al. ARPE-19, A human retinal pigment epithelial cell line with differentiated properties. Exp. Eye Res. 62: 155-169, 1996. PubMed: 8698076 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |