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微信小章| 产品名称： | anti-130-kDa Mesothelial-Ciliated Cells |
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| 商品货号： | TS212670 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The hybridoma cell line anti-130-kDa Mesothelial-Ciliated Cells produces an antibody (IgM; kappa light chain)) that is specific for the 130-kDa mesothelial and ciliated cell protein in human and rat lungs. In formalin-fixed, paraffin-embedded tissues from adult human and rat organs, the antibody specifically stained the luminal/apical surfaces of pulmonary and nonpulmonary ciliated and mesothelial cells. The antibody may be useful in the diagnosis of epithelial
mesotheliomas and ovarian/endometrial carcinomas, because it selectively stains these tumors and is reactive with formalin-fixed, paraffin-embedded tissues. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The surfactant was isolated by lung lavage from cadaveric lungs and processed to yield surfactant binding proteins. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against the 130-kDa mesothelial and ciliated cell protein |
| Cellular Products： | immunoglobulin; monoclonal antibody; against the 130-kDa mesothelial and ciliated cell protein |
| Comments： | The hybridoma cell line anti-130-kDa Mesothelial-Ciliated Cells produces an antibody (IgM; kappa light chain)) that is specific for the 130-kDa mesothelial and ciliated cell protein in human and rat lungs. By Western blotting, the antibody stained a protein of about 130 kDa. In formalin-fixed, paraffin-embedded tissues from adult human and rat organs, the antibody specifically stained the luminal/apical surfaces of pulmonary and nonpulmonary ciliated and mesothelial cells. Airway cell staining was detectable in human fetal lungs at 12 weeks of gestation and at Day 18 of gestation in fetal rat. The antibody reacted with human and rat fetal mesothelial cells at the gestational ages of 15 weeks and 17 days, respectively. It also stained ciliated cells in endosalpinx and endometrium. The antibody may be useful in the diagnosis of epithelial
mesotheliomas and ovarian/endometrial carcinomas, because it selectively stains these tumors and is reactive with formalin-fixed, paraffin-embedded tissues. The cell line was formed by the fusion of P3/X63/Ag mouse myeloma cells with splenocytes from a BALB/c mouse inoculated with surfactant binding proteins. The surfactant was isolated by lung lavage from cadaveric lungs and processed to yield surfactant binding proteins. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2xa0to 3 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106xa0 viable cells/mL. Add fresh medium as cell density increases. Medium Renewal:xa0Every 2 to 3 days |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgM, kappa light chain |
| Name of Depositor： | G Singh |
| Deposited As： | Mus musculus (B cell); Mus musculus (myeloma) |
| References： | Singh G, et al. Expression of a 130-kDa mesothelial and ciliated cell Ag (MCp130) in normal and developing human and rat lung and its role as a diagnostic marker for mesotheliomas and tumors of the female reproductive system. Lab. Invest. 73: 48-58, 1995. PubMed: 7603040 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |