微信小陈
微信小章| 产品名称： | Antheraea cells |
|---|---|
| 商品货号： | TS212673 |
| Organism： | Antheraea eucalypti, moth |
| Tissue： | ovary |
| Product Format： | frozen |
| Morphology： | spindle, round and crescent shaped cells |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | female |
| Applications： | This cell line derived from ovarian tissues of the moth, Antheraea eucalypti, by Grace in 1962, constituted the first true line of arthropod cells established in cell culture. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This cell line derived from ovarian tissues of the moth, Antheraea eucalypti, by Grace in 1962, constituted the first true line of arthropod cells established in cell culture. Because of the difficulty and expense in obtaining significant volumes of lepidopteran hemolymph, the Antheraea cells were adapted to hemolymph-free culture medium by Yunker, Vaughn
and Cory . The cell line was grown in Graces insect tissue culture medium supplemented with 10% FBS (heat-inactivated), 10% whole chicken egg ultrafiltrate and 1% bovine plasma albumin (fraction V). The cells grow predominantly in suspension although some cells adhere to the vessel walls. Yunker and Cory found the adapted Antheraea cells were able to support the growth of a number of arboviruses. |
| Clinical Data： | female |
| Virus Resistance： | poliovirus 1; herpes simplex; vaccinia |
| Comments： | This cell line derived from ovarian tissues of the moth, Antheraea eucalypti, by Grace in 1962, constituted the first true line of arthropod cells established in cell culture. Because of the difficulty and expense in obtaining significant volumes of lepidopteran hemolymph, the Antheraea cells were adapted to hemolymph-free culture medium by Yunker, Vaughn
and Cory . The cell line was grown in Graces insect tissue culture medium supplemented with 10% FBS (heat-inactivated), 10% whole chicken egg ultrafiltrate and 1% bovine plasma albumin (fraction V). The cells grow predominantly in suspension although some cells adhere to the vessel walls. Yunker and Cory found the adapted Antheraea cells were able to support the growth of a number of arboviruses. |
| Complete Growth Medium： | Graces insect medium, 79%; heat-inactivated fetal bovine serum, 10%; whole egg ultrafiltrate, 10%; bovine plasma albumin (fraction V), 1%
|
| Subculturing： | The cells grow in suspension and subcultures are prepared by diluting 0.5 mL of cell suspension with 4.5 mL of fresh culture medium and dispensing into a new flask. Culture at 23°C to 25°C. Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:12 is recommended |
| Cryopreservation： | Complete growth medium described above supplemented with 10% (v/v) glycerol. |
| Culture Conditions： | Temperature: 24°C Atmosphere: 100% Air, without Carbon dioxide (CO2) |
| Name of Depositor： | JL Vaughn |
| Deposited As： | Antheraea eucalypti |
| Year of Origin： | 1962 |
| References： | . . Nature 195: 1, 1962. Yunker CE, et al. Adaptation of an insect cell line (Graces Antheraea cells) to medium free of insect hemolymph. Science 155: 1565-1566, 1967. PubMed: 6020481 Yunker CE, Cory J. Infection of Graces Antheraea cells with Arboviruses. Am. J. Trop. Med. Hyg. 17: 889-893, 1968. PubMed: 5726135 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |