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微信小章| 产品名称： | AN3 CA |
|---|---|
| 商品货号： | TS212674 |
| Organism： | Homo sapiens, human |
| Tissue： | uterus; endometrium |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenocarcinoma |
| Age： | 55 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | 55 years Caucasian female |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice Yes, in the cheek pouch of cortisone treated hamsters |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 sq. cm flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X
CSF1PO: 13*
D13S317: 12,14
D16S539: 10,14
D5S818: 11,14
D7S820: 7*,10,7.1
THO1: 10,9.3*
TPOX: 8,10
vWA: 14,20
*Note: This cell line has historically exhibited instability at CSF1PO 13, D7S820 7, and THO1 9.3 |
| Isoenzymes： | AK-1, 1-2 ES-D, 1 G6PD, B GLO-I, 2 PGM1, 1 PGM3, 1-2 |
| Name of Depositor： | CJ Dawe |
| Deposited As： | Homo sapiens |
| References： | Dawe CJ, et al. Growth in continuous culture, and in hamsters, of cells from a neoplasma assoicated with Acanthosis nigricans. J. Natl. Cancer Inst. 33: 441-456, 1964. PubMed: 14207855 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105 The cells produce undifferentiated malignant tumors. at low frequency (22%) C. J. Dawe and associates derived this cell line from a metastatic lesion in the lymph node of a patient with endometrial carcinoma alerted to the condition by onset of the malignant disorder acanthosis nigricans. |