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微信小章| 产品名称： | anti-SR (1H4) |
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| 商品货号： | TS212675 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with the family of 6 essential splicing factors, the SR proteins. Specificity is similar to Mab104 (ATCC CRL-2067) but because it is an IgG may be potentially more useful than Mab104 which is an IgM. This antibody may be useful to determine whether a protein studied for its interactions with RNA is a SR protein and it is also useful in Western Blot, immunoprecipitation, and cytological assays. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with mass-isolated nuclei from the oocytes of Xenopus laevis. Spleen cells were fused with FOX-NY myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against SR proteins (pre-mRNA splicing factors) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against SR proteins (pre-mRNA splicing factors)
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| Comments： | Animals were immunized with mass-isolated nuclei from the oocytes of Xenopus laevis. Spleen cells were fused with FOX-NY myeloma cells. The antibody reacts with the family of 6 essential splicing factors, the SR proteins. SR proteins are a family of proteins that have pre-mRNA splicing activity (SR comes from the sequences of consecutive serine and arginine residues in these proteins). The family consists of at least 6 proteins with approximate masses of 75000, 55000, 40000, two at 30000 and 20000 daltons. Specificity is similar to Mab104 (ATCC CRL-2067) but because it is an IgG may be potentially more useful than Mab104 which is an IgM. This antibody may be useful to determine whether a protein studied for its interactions with RNA is a SR protein and it is also useful in Western Blot, immunoprecipitation, and cytological assays. |
| Complete Growth Medium： | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.075 mM adenine, 800 nM aminopterin, 0.016 mM thymidine (AAT) and 20% fetal bovine serum
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| Subculturing： | Cultures can be maintained by addition of fresh medium or replacement of medium. Alternately, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL.xa0 Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL. Medium Renewal:xa0Every 2 to 3 days |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG1 |
| Name of Depositor： | KM Neugebauer, MB Roth |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Neugebauer KM, Roth MB. Distribution of pre-mRNA splicing factors at sites of RNA polymerase II transcription. Genes Dev. 11: 1148-1159, 1997. PubMed: 9159396 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |