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| 产品名称： | alphaM346C7C1 aM346C7C1, anti M346C7C1 |
|---|---|
| 商品货号： | TS212681 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | Strain:xa0 BALB/c (B cell); BALB/c (myeloma) |
| Applications： | The antibody reacts with 2134P fimbriae of enterotoxigenic Escherichia coli. |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | Spleen cells were fused with Sp2/0 mouse myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against 2134P fimbriae of enterotoxigenic Escherichia coli |
| Cellular Products： | immunoglobulin; monoclonal antibody; against 2134P fimbriae of enterotoxigenic Escherichia coli |
| Comments： | Animals were immunized with a crude extract containing the 2134P antigen prepaed from Escherichia coli, strain 2134 bacteria. Spleen cells were fused with Sp2/0 mouse myeloma cells. The antibody reacts with 2134P fimbriae of enterotoxigenic Escherichia coli. The antibody blocks the binding of 2134P+ bacteria to intestinal epithelial cells. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose and 2 mM L-glutamine, 92.5%; horse serum, 5%; bovine calf serum, 2.5%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
| Isotype： | IgG1 |
| Name of Depositor： | National Animal Disease Center, ARS, USDA |
| References： | Dean-Nystrom EA, et al. A monoclonal antibody identifies 2134P fimbriae as adhesins on enterotoxigenic Escherichia coli isolated from postweaning pigs. Vet. Microbiol. 37: 101-114, 1993. PubMed: 7905220 |