微信小陈
微信小章| 产品名称： | Amdur II |
|---|---|
| 商品货号： | TS212682 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | methylmalonic acidemia |
| Age： | 1 year |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | normal human male; stable |
| Comments： | The cells have defect in propionic acid metabolism and accumulate methylmalonic acid. The cells have a life expectancy of at least 40 serial passages. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:3 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Complete culture medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C Atmosphere: Air, 95%; CO2, 5% |
| Isoenzymes： | G6PD, B |
| Name of Depositor： | WJ Mellman |
| Deposited As： | Homo sapiens |
| Passage History： | The cells have a life expectancy of at least 40 serial passages. |
| References： | Morrow G, et al. Propionate metabolism in cells cultured from a patient with methylmalonic acidemia. Pediatr. Res. 3: 217-219, 1969. PubMed: 5788784 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |