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微信小章| 产品名称： | AMJ2-C11 |
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| 商品货号： | TS212687 |
| Organism： | Mus musculus, mouse |
| Tissue： | lung |
| Cell Type： | macrophage (alveolar); infected with J2 virus |
| Product Format： | frozen |
| Morphology： | macrophage |
| Culture Properties： | suspension, with some loosely adherent cells |
| Biosafety Level： | 2 xa0Cells contain J2 murine retrovirus viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 10 weeks old |
| Gender： | female |
| Strain： | C57BL/6J |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | AMJ2-C8 (ATCC CRL-2455) and AMJ2-C11 (TS212687) are cloned, continuous, alveolar macrophage (AM) cell lines generated from C57BL6J mice by in vitro infection with the J2 retrovirus carrying the v-raf and v-myc oncogenes. |
| Clinical Data： | female
10 weeks old |
| Antigen Expression： | MAC-1 (CD11b) +; MAC-2 +; Fc receptor (FcR) +; Ly-5 +; Thy-1 -; Lyt-1 - |
| Genes Expressed： | interleukin-6 (interleukin 6, IL-6) |
| Cellular Products： | interleukin-6 (interleukin 6, IL-6) |
| Comments： | AMJ2-C8 (ATCC CRL-2455) and AMJ2-C11 (TS212687) are cloned, continuous, alveolar macrophage (AM) cell lines generated from C57BL6J mice by in vitro infection with the J2 retrovirus carrying the v-raf and v-myc oncogenes. Flow cytometry detected the product of the raf gene in the cytoplasm of these cell lines. Studies on the tumoricidal properties of these cell lines demonstrated differences in their response to a panel of known macrophage activators. AMJ2-C8 was activated following exposure to recombinant murine interferon gamma (rMuIFN-gamma) but not lipopolysaccharide (LPS) or muramyl dipeptide (MDP). AMJ2-C11 most closely resembled the response pattern of the parental AM, since it could be activated by either the combination of rMuIFN-gamma plus LPS or rMuIFN-gamma plus MDP. The cells retain many characteristics of alveolar macrophages. They are phagocytic, non-specific esterase positive and they express macrophage Mac-1 antigens and Fc receptors. Constitutive expression of MHC-class-II antigens was low on AMJ2-C11 but was increased following exposure to rMuIFN-gamma. The cell line did not secrete substantial amounts of IL-1 or TNF but did secrete large amounts of IL-6. The cells produce nitric oxide (NO) when stimulated with a mixture of rMuIFN-gamma and LPS.
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| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 5 mM HEPES, 95%; fetal bovine serum, 5%
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| Subculturing： | Firmly tap the flask against palm of hand to dislodge any attached cells and transfer along with the floating cells into new flasks. Medium Renewal: Twice per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with an additional 5% fetal bovine serum and 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor： | AV Palleroni |
| Deposited As： | mouse |
| References： | Palleroni AV, et al. Tumoricidal alveolar macrophage and tumor infiltrating macrophage cell lines. Int. J. Cancer 49: 296-302, 1991. PubMed: 1879973 Palleroni AV, et al. Nitric oxide synthase induction in lines of macrophages from different anatomical sites. Cell. Mol. Biol. 44: 527-535, 1998. PubMed: 9620450 |