| 产品名称: | AKR1.G.1.OUAR.1.26 |
|---|---|
| 商品货号: | TS212694 |
| Organism: | Mus musculus, mouse |
| Tissue: | thymus |
| Product Format: | frozen |
| Morphology: | lymphoblast |
| Culture Properties: | suspension |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease: | lymphoma |
| Strain: | AKR/J |
| Applications: | The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain. The line may be useful for fusion studies and as a host for the transfection of cloned genes. |
| Antigen Expression: | Thy-1; L3T4; Lyt-2; HSA |
| Genes Expressed: | Thy-1; L3T4; Lyt-2; HSA |
| Comments: | The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain. The line may be useful for fusion studies and as a host for the transfection of cloned genes. The cells have the surface antigen properties of cortical thymocytes. |
| Complete Growth Medium: | Dulbeccos modified Eagles medium, 90%; heat-inactivated horse serum, 10%
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Name of Depositor: | R Hyman |
| Deposited As: | Mus musculus |
| References: | . . Immunogenetics 10: 261-271, 1980. |