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| 产品名称： | alpha BL5C2.870005 870005, aBL5C2.870005 |
|---|---|
| 商品货号： | TS212696 |
| Organism： | bovine (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody neutralizes BHV-1 and is protective against BHV-1 induced mortality in rabbits. |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | Lymph node cells were fused with Sp2/0 mouse myeloma cells. Initial hybrids were refused with additional lymph node cells from the same animal and reselected. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against bovine herpesvirus 1 (BHV-1) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against bovine herpesvirus 1 (BHV-1) |
| Comments： | Animals were immunized with live BHV-1. Lymph node cells were fused with Sp2/0 mouse myeloma cells. Initial hybrids were refused with additional lymph node cells from the same animal and reselected. The antibody neutralizes BHV-1 and is protective against BHV-1 induced mortality in rabbits. |
| Complete Growth Medium： | Culture Medium: Dulbeccos modified Eagles medium with 4.5 g/L glucose, 90%; horse serum, 10%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | fetal bovine serum, 85%; DMSO, 15% |
| Isotype： | IgG1 |
| Name of Depositor： | The United States of America |
| U.S. Patent Number： | |
| References： | Levings RL, Stoll IR. Bovine monoclonal antibodies to bovine herpesvirus I from sequential fusion heterohybridomas. US Patent 5,026,646 dated Jun 25 1991 |