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微信小章| 产品名称： | Aedes albopictus clone C6/36 |
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| 商品货号： | TS212698 |
| Organism： | Aedes albopictus, mosquito, Asian tiger |
| Tissue： | larva, whole |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | larva |
| Applications： | This cell line is useful for the replication of flaviviruses and reportedly can be used to replicate Dengue viruses to high titers. They are also a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | diploid |
| Images： |  |
| Tumorigenic： | No |
| Effects： | Yes, did form colonies in semisolid medium No, the cells were not tumorigenic in immunosuppressed mice |
| Comments： | The cells are non-anchorage dependent, are not tumorigenic and maintain a diploid chromosome number. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Subcultures are prepared by scraping or by vigorous pipetting. Remove the old medium, add fresh complete culture medium, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Twice per week |
| Cryopreservation： | Freeze medium: culture medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 28°C |
| Name of Depositor： | KH Eckels |
| Deposited As： | Aedes albopictus |
| References： | Igarashi A. Isolation of a Singhs Aedes albopictus cell clone sensitive to Dengue and Chikungunya viruses. J. Gen. Virol. 40: 531-544, 1978. PubMed: 690610 Singh KRP. Cell cultures derived from larvae of Aedes albopictus (Skuse) and Aedes aegypti (L.). Curr. Sci. 36: 506-508, 1967. Clone C6/36 was derived from A. albopictus cells (see ATCC CCL-126) which were adapted to Eagles minimum essential medium by A. Igarashi and then cloned and recloned by seeding single cell suspensions into petri dishes. |