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微信小章| 产品名称： | Aedes aegypti ATC-10 |
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| 商品货号： | TS212701 |
| Organism： | Aedes aegypti, mosquito |
| Tissue： | larva |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | larva |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The Aedes aegypti ATC-10 cell line was established by K.R.P. Singh from a pool of several hundred freshly hatched larvae. |
| Virus Susceptibility： | Chandipura virus
Chikungunya virus West Nile virus , West Nile virus Vesicular stomatitis virus |
| Comments： | Arbovirus studies have shown that the Aedes aegypti ATC-10 cells are susceptible to Chikungunga, West Nile and Chandipura viruses. |
| Complete Growth Medium： | Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids and 1.0 mM sodium pyruvate, 80%; heat-inactivated fetal bovine serum, 20%
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005. |
| Culture Conditions： | Temperature: 28°C; (Max. 30°C, Min. 27°C)
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | SM Buckley |
| Deposited As： | Aedes aegypti |
| References： | Singh KRP. Cell cultures derived from larvae of Aedes albopictus (Skuse) and Aedes aegypti (L.). Curr. Sci. 36: 506-508, 1967. Singh KRP, Paul SD. Multiplication of arboviruses in cell lines from from Aedes albopictus and Aedes aegypti. Curr. Sci. 37: 65-67, 1968. Singh KR. Propagation of arboviruses in Singhs Aedes cell lines. I. Growth of arboviruses in Aedes albopictus and A. aegypti cell lines. Curr. Top. Microbiol. Immunol. 55: 127-133, 1971. PubMed: 5142320 Mitsuhashi J, Maramorosch K. Leafhopper tissue culture: Embryonic, nymphal and imaginal tissues from aseptic insects. Contrib. Boyce Thompson Inst. 22: 435-460, 1964. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |