微信小陈
微信小章| 产品名称： | Aedes albopictus ATC-15 |
|---|---|
| 商品货号： | TS212704 |
| Organism： | Aedes albopictus, mosquito, Asian tiger |
| Tissue： | larva |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | larva |
| Applications： | transfection host |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | Karyotype stable within diploid stemline number. The chromosomes have median centromeres. In a few cases, a chromosome with a submedian centromere was noted. Two (2) cells with chromosome breaks (3%) and 6 cells with secondary constrictions (10%). |
| Images： |  |
| Derivation： | The Aedes albopictus ATC-15 cell line was established from a pool of several hundred freshly hatched larvae. |
| Virus Susceptibility： | Dengue virus type 1
Dengue virus type 2 Dengue virus type 3 Colorado tick fever virus Dengue virus type 4 Japanese encephalitis virus , Japanese encephalitis virus Human poliovirus 1 Human herpesvirus 1 , Herpes simplex virus 1 Vaccinia virus Vesicular stomatitis virus |
| Complete Growth Medium： | Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids and 1.0 mM sodium pyruvate, 80%; heat-inactivated fetal bovine serum, 20%
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:3 to 1:6xa0
Medium Renewal: 1 to 2 times per week
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium supplemented with 10% (v/v) DMSO.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
|
| Culture Conditions： | Temperature: 28°C; (Max. 28°C, Min. 26°C)
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Population Doubling Time： | 26 |
| Name of Depositor： | SM Buckley |
| Deposited As： | Aedes albopictus |
| References： | Singh KRP. Cell cultures derived from larvae of Aedes albopictus (Skuse) and Aedes aegypti (L.). Curr. Sci. 36: 506-508, 1967. Singh KRP, Paul SD. Multiplication of arboviruses in cell lines from from Aedes albopictus and Aedes aegypti. Curr. Sci. 37: 65-67, 1968. Singh KR. Propagation of arboviruses in Singhs Aedes cell lines. I. Growth of arboviruses in Aedes albopictus and A. aegypti cell lines. Curr. Top. Microbiol. Immunol. 55: 127-133, 1971. PubMed: 5142320 Mitsuhashi J, Maramorosch K. Leafhopper tissue culture: Embryonic, nymphal and imaginal tissues from aseptic insects. Contrib. Boyce Thompson Inst. 22: 435-460, 1964. Buckley SM. Susceptibility of the Aedes albopictus and A. aegypti cell lines to infection with arboviruses. Proc. Soc. Exp. Biol. Med. 131: 625-630, 1969. PubMed: 5787147 Yunker CE, Cory J. Colorado tick fever virus: growth in a mosquito cell line. J. Virol. 3: 631-632, 1969. PubMed: 5798247 tissue from freshly hatched larvae Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |