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| 产品名称： | AB1-2 |
|---|---|
| 商品货号： | TS212713 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue： | lymph node |
| Cell Type： | hybridoma:lymphoblast B lymphocyte; somatic cell hybri |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | A/J |
| Applications： | The antibody induces neonatal suppression, inhibits plaque forming cell induction by phosphorylcholine (PC) antigens and specifically inhibits anti-PC plaque forming cells. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Animals were immunized with purified immunoglobulins from the MOPC 460, MOPC 5558 and HOPC 8 myelomas. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against T15 idiotype |
| Cellular Products： | immunoglobulin; monoclonal antibody; against T15 idiotype |
| Comments： | Animals were immunized with purified immunoglobulins from the MOPC 460, MOPC 5558 and HOPC 8 myelomas. The antibody induces neonatal suppression, inhibits plaque forming cell induction by phosphorylcholine (PC) antigens and specifically inhibits anti-PC plaque forming cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | mouse IgG1 kappa , IgG1; kappa light chain |
| Name of Depositor： | JF Kearney |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Kearney JF, et al. Monoclonal vs. heterogeneous anti-H-8 antibodies in the analysis of the anti-phosphorylcholine response in BALB/c mice. Eur. J. Immunol. 11: 877-883, 1981. PubMed: 7035186 |