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| 产品名称： | ACT IV |
|---|---|
| 商品货号： | TS212717 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with actin from prokaryotic organisms at an epitope distinct from that recognized by ACT I (ATCC HB-80). Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against actin |
| Cellular Products： | immunoglobulin; monoclonal antibody; against actin |
| Comments： | Animals were immunized with actin from Dictyostelium discoideum. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with actin from prokaryotic organisms at an epitope distinct from that recognized by ACT I (ATCC HB-80). The antibody does not cross-react with eukaryotic actins. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | RPMI 1640 medium with 0.1 mM hypoxanthine, 400 nM aminopterin and 0.016 mM thymidine, 80%; fetal bovine serum, 20%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG1 |
| Name of Depositor： | P Parham |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Simpson PA, et al. Monoclonal antibodies prepared against Dictyostelium actin: characterization and interactions with actin. J. Cell Biol. 99: 287-295, 1984. PubMed: 6203918 |