微信小陈
微信小章
产品简介
购买须知
| 产品名称： | A9 |
|---|---|
| 商品货号： | TS212720 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Cell membranes used for immunization derived from UT-1, a cell line derived from the CHO K1 cell line.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | Immunoglobulin; monoclonal antibody; against HMG-CoA reductase (3-hydroxy-3-methyl-glutaryl Coenzyme A reductase, HMG CoA reductase) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against HMG-CoA reductase (3-hydroxy-3-methyl-glutaryl Coenzyme A reductase, HMG CoA reductase) |
| Tumorigenic： | Yes |
| Effects： | Yes, in pristane primed BALB/c mice |
| Comments： | Animals were immunized with cell membranes from UT-1. The antibody reacts with HMG-CoA reductase (the rate limiting enzyme in the cholesterol biosynthetic pathway). Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL.xa0 Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL. Medium Renewal: Every 2 to 3 days |
| Isotype： | IgG1 |
| Name of Depositor： | JL Goldstein, YK Ho |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Liscum L, et al. Regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase and its mRNA in rat liver as studied with a monoclonal antibody and a cDNA probe. J. Biol. Chem. 258: 8450-8455, 1983. PubMed: 6688077 |