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微信小章| 产品名称： | A-704 |
|---|---|
| 商品货号： | TS212721 |
| Organism： | Homo sapiens, human |
| Tissue： | kidney |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenocarcinoma |
| Age： | 78 years |
| Gender： | male |
| Applications： | This cell line is a suitable transfection host. ref |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | (P59) diploid to hyperdiploid, hypertriploid to hypertetraploid with abnormalities including breaks, dicentrics and endoreduplication |
| Derivation： | The A-704 line was derived by D.J. Giard as indicated in the description for ATCC HTB-41. |
| Clinical Data： | male |
| Tumorigenic： | No |
| Effects： | No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| STR Profile： | Amelogenin: X,Y CSF1PO: 7,8 D13S317: 8 D16S539: 12,13 D5S818: 10,11 D7S820: 10 THO1: 7,9 TPOX: 11 vWA: 14,18 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1 PGM3, 1-2 |
| Name of Depositor： | DJ Giard |
| Deposited As： | Homo sapiens |
| References： | Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758 Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047 |
| Cross References： | Nucleotide (GenBank) : U02327 Human clone ndf19 neu differentiation factor mRNA, partial cds. Nucleotide (GenBank) : U02330 Human clone ndf33 neu differentiation factor mRNA, partial cds. Nucleotide (GenBank) : U02326 Human clone ndf43 neu differentiation factor mRNA, complete cds. |