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微信小章| 产品名称： | A9 (APRT and HPRT negative derivative of Strain L) |
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| 商品货号： | TS212723 |
| Organism： | Mus musculus, mouse |
| Tissue： | subcutaneous connective tissue; areolar and adipose |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | 100 days |
| Gender： | male |
| Strain： | C3H/An |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 52; range = 46 to 54; This is a hyperdiploid cell line with 24 to 26 marker chromosomes common to all cells., Nine markers (M1 - M9) are common to L-M(TK-) (see ATCC CCL-1.3) and 16 to 17 are found only in A9. The latter markers include Rb(3.5), Rb(4.15), Rb(5.5), T(8;19), Rb(9.17) and 11 to 12 others., Structurally normal chromosomes 4, 15, 16, 17, 18 and X were absent. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
| Derivation： | The A9 cell line was isolated by J.W. Littlefield from wild-type Strain L cells. |
| Clinical Data： | male |
| Antigen Expression： | H-2k |
| Tumorigenic： | Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10exp7 cells. |
| Effects： | Yes, in nude mice |
| Comments： | The cells are sensitive to HAT selection medium and deficient in adenosine phosphoribosyl transferase (APRT) and hypoxanthine phosphoribosyl transferase (HPRT).
The cells are resistant to 8-azaguanine (0.003 mg/mL) and 6-thioguanine (0.1 mM). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5g/L glucose, 90%; fetal bovine serum, 10%
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: Every 2 to 3 days
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| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor： | TR Chen |
| Deposited As： | Mus musculus |
| References： | Littlefield JW. The inosinic acid pyrophosphorylase activity of mouse fibroblasts partially resistant to 8-azaguanine. Proc. Natl. Acad. Sci. USA 50: 568-573, 1963. PubMed: 14067106 Chen TR. Cytogenetics of somatic cell hybrids. II. Modal karyotypes and mosaicism in mouse mutant cell lines: LM(tk-), A9, and Rag. Cancer Genet. Cytogenet. 24: 95-107, 1987. PubMed: 3466673 Mayr GA, Freimuth P. A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365 |