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微信小章| 产品名称： | A-72 |
|---|---|
| 商品货号： | TS212724 |
| Organism： | Canis familiaris, dog |
| Tissue： | unknown |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | tumor |
| Age： | 8 years |
| Gender： | female |
| Strain： | Golden retriever |
| Applications： | efficacy testing Ref |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was established from a 1 cm diameter tumor taken from the left thigh of a female Golden Retriever. |
| Clinical Data： | female |
| Virus Susceptibility： | Canine adenovirus 2
Canine coronavirus , Canine coronavirus Canine parainfluenza virus Canid herpesvirus 1 Canine distemper virus Canine minute virus |
| Comments： | A canine parvovirus related to Feline panleukopenia virus has been recovered from A-72 cells. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Remove medium, rinse with fresh 0.25% trypsin, 0.53 mM EDTA solution, remove trypsin and let the culture sit at 37°C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
|
| Cryopreservation： | Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 100%
Temperature: 37°C |
| Name of Depositor： | LN Binn |
| Deposited As： | Canis familiaris |
| References： | Binn LN, et al. Establishment of a canine cell line: derivation, characterization, and viral spectrum. Am. J. Vet. Res. 41: 855-860, 1980. PubMed: 6254399 The line was established from a 1 cm diameter tumor taken from the left thigh of a female Golden Retriever. Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2197-02. |
Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2197-02.