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微信小章| 产品名称： | AA8 |
|---|---|
| 商品货号： | TS212727 |
| Organism： | Cricetulus griseus, hamster, Chinese |
| Tissue： | ovary |
| Product Format： | frozen |
| Morphology： | epithelial-like |
| Culture Properties： | monolayer and suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | female |
| Applications： | The line is useful for gene mutation assays and is the parent line for a series of repair deficient mutants (see ATCC CRL-1860, ATCC CRL-1861, ATCC CRL-1862, ATCC CRL-1865, ATCC CRL-1866 and ATCC CRL-1867). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | chromosome number = 21 |
| Derivation： | This line is a derivative of the CHO cell line. |
| Clinical Data： | female |
| Comments： | This line is a derivative of the CHO cell line.
The AA8 line has 21 chromosomes and is heterozygous at the aprt locus.
The line is useful for gene mutation assays and is the parent line for a series of repair deficient mutants (see ATCC CRL-1860, ATCC CRL-1861, ATCC CRL-1862, ATCC CRL-1865, ATCC CRL-1866 and ATCC CRL-1867). |
| Complete Growth Medium： | Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:4 to 1:12 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Population Doubling Time： | 12 hrs |
| Name of Depositor： | LH Thompson |
| References： | Thompson LH, et al. Validation of conditions for efficient detection of HPRT and APRT mutations in suspension-cultured Chinese hamster ovary cells. Mutat. Res. 74: 21-36, 1980. PubMed: 7360155 Bessho T, et al. Initiation of DNA interstrand cross-link repair in humans: the nucleotide excision repair system makes dual incisions 5" to the cross-linked base and removes a 22- to 28-nucleotide-long damage-free strand. Mol. Cell. Biol. 17: 6822-6830, 1997. PubMed: 9372913 Reardon JT, et al. Isolation and characterization of two human transcription factor IIH (TFIIH)-related complexes: ERCC2/CAKand TFIIH. Proc. Natl. Acad. Sci. USA 93: 6482-6487, 1996. PubMed: 8692841 Busch D, et al. Summary of complementation groups of UV-sensitive CHO cell mutants isolated by large-scale screening. Mutagenesis 4: 349-354, 1989. PubMed: 2687628 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |