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微信小章| 产品名称： | A-498 |
|---|---|
| 商品货号： | TS212730 |
| Organism： | Homo sapiens, human |
| Tissue： | kidney |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | carcinoma |
| Age： | 52 years |
| Gender： | female |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | S. Aaronson isolated this line using techniques as described for ATCC HTB-41. |
| Clinical Data： | female |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice; forms undifferentiated carcinoma; also forms tumors in anti thymocyte serum treated newborn mice |
| Comments： | |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： |
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Twice per week |
| Cryopreservation： | Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 11,12 D13S317: 12 D16S539: 12 D5S818: 11,13 D7S820: 10,11 THO1: 6,9.3 TPOX: 8,11 vWA: 18 |
| Isoenzymes： | AK-1, 1 ES-D, 2 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1-2 PGM3, 1 |
| Name of Depositor： | W Nelson-Rees |
| Deposited As： | Homo sapiens |
| References： | Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216 Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758 Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047 |