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| 产品名称： | A5.4 1506 A5.4 |
|---|---|
| 商品货号： | TS212731 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody binds to an epitope in amino acids 113 through 243 on human ApoA-I. It is useful for detecting ApoA-I in immunoblotting applications. |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human apolipoprotein A-I (Apo-A-I) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human apolipoprotein A-I (Apo-A-I) |
| Tumorigenic： | Yes |
| Effects： | Yes, forms ascites in pristane primed BALB/c mice |
| Comments： | Animals were immunized with the major protein peak (G-200 fractionation) of delipidated human high density lipoprotein (HDL). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody binds to an epitope in amino acids 113 through 243 on human ApoA-I. It is useful for detecting ApoA-I in immunoblotting applications. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose, 0.1 mM non-essential amino acids and 1 mM sodium pyruvate, 85%; horse serum, 10%; fetal bovine serum, 5%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Isotype： | IgG2a |
| Name of Depositor： | G Schonfeld |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Jonas A, et al. Apolipoprotein A-I structure and lipid properties in homogeneous, reconstituted spherical and discoidal high density lipoproteins. J. Biol. Chem. 265: 22123-22129, 1990. PubMed: 2125044 |