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| 产品名称： | A6 |
|---|---|
| 商品货号： | TS212737 |
| Organism： | Xenopus laevis, frog, South African clawed |
| Tissue： | kidney |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | adult |
| Gender： | male |
| Clinical Data： | male |
| Virus Resistance： | poliovirus 1; vesicular stomatitis (Indiana); herpes simplex; vaccinia; pseudorabies |
| Complete Growth Medium： | NCTC 109 medium, 75%; distilled water, 15%; fetal bovine serum, 10%
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Twice per week Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 26°C |
| Name of Depositor： | KA Rafferty |
| Deposited As： | Xenopus laevis |
| References： | Mizell, M. Biology of amphibian tumors. New York: Springer-Verlag; 1969. Rokaw MD, et al. Regulation of a sodium channel-associated G-protein by aldosterone. J. Biol. Chem. 271: 4491-4496, 1996. PubMed: 8626803 |