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| 产品名称： | A3.4H2 |
|---|---|
| 商品货号： | TS212739 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | Strain: BALB/c (B cell); BALB/c (myeloma) |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human CTLA-4 (CD152) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human CTLA-4 (CD152) |
| Comments： | Animals were immunized with activated human T cell clones. Spleen cells were fused with Sp2/0-Ag14 cells. The antibody was able to block B7 ligand binding. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Add media or fluid change as cell concentration increases. (Cells attach firmly to flask surface). Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG2a; kappa light chain |
| Name of Depositor： | VK Kuchroo, EA Greenfield |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| U.S. Patent Number： | |
| References： | Kuchroo VK, Greenfield EA. Specific antibodies and antibody fragments. US Patent 6,207,156 dated Mar 27 2001 |