微信小陈
微信小章
产品简介
购买须知
| 产品名称： | A2E11 |
|---|---|
| 商品货号： | TS212741 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase HPRT and adenine phosphoribosyl transferase APRT). The antibody is highly specific for aldosterone and does not cross-react significantly with other steroids. |
| Derivation： | Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase HPRT and adenine phosphoribosyl transferase APRT). |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against aldosterone |
| Cellular Products： | immunoglobulin; monoclonal antibody; against aldosterone |
| Comments： | Animals were immunized with a BSA conjugate of the 3-O carboxylmethyl oxime of aldosterone. Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase HPRT and adenine phosphoribosyl transferase APRT). The antibody is highly specific for aldosterone and does not cross-react significantly with other steroids. |
| Complete Growth Medium： | Iscoves modified Dulbeccos medium with 1 mM sodium pyruvate, 0.05 mM 2-mercaptoethanol and 1 mM L-glutamine, 90%; fetal bovine serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG1 |
| Name of Depositor： | CE Gomez-Sanchez |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Gomez-Sanchez CE, et al. The production of monoclonal antibodies against aldosterone. Steroids 49: 581-587, 1987. PubMed: 3453564 |