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微信小章| 产品名称： | A-375 A375 |
|---|---|
| 商品货号： | TS212742 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | malignant melanoma |
| Age： | 54 years |
| Gender： | female |
| Applications： | This cell line is a suitable transfection host. This cell line is also the ideal control for NRAS mutant-A375xa0isogenic cell line (ATCC® CRL-1619IG-2™). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | It is a hypotriploid with a modal number of 62 chromosomes. There are 9 marker chromosomes that are commonly found in each cell, and normal N2, N6, and N22 are present at one copy per cell. |
| Images： |  |
| Clinical Data： | 54 years
female |
| Tumorigenic： | Yes |
| Effects： | Yes, in immunosuppressed mice |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.xa0Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 11,12 D13S317: 11,14 D16S539: 9 D5S818: 12 D7S820: 9 THO1: 8 TPOX: 8,10 vWA: 16,17 |
| Name of Depositor： | DJ Giard |
| Deposited As： | Homo sapiens |
| References： | Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758 Gershwin ME, et al. Immunobiology of heterotransplanted human tumors in nude mice. J. Natl. Cancer Inst. 58: 1455-1463, 1977. PubMed: 857033 Cormier JN, et al. Heterogeneous expression of melanoma-associated antigens and HLA-A2 in metastatic melanoma in vivo. Int. J. Cancer 75: 517-524, 1998. PubMed: 9466650 Sharma SD, et al. Melanotropic peptide-conjugated beads for microscopic visualization and characterization of melanoma melanotropin receptors. Proc. Natl. Acad. Sci. USA 93: 13715-13720, 1996. PubMed: 8943000 |