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| 产品名称： | A11.1 M |
|---|---|
| 商品货号： | TS212750 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Animals were immunized with Epstein-Barr virus transformed human B cells (HLA phenotype A11,A24,B27,B44,Bw4,Cw2). Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against HLA A11, A24; cytotoxic |
| Cellular Products： | immunoglobulin; monoclonal antibody; against HLA A11, A24; cytotoxic |
| Comments： | Animals were immunized with Epstein-Barr virus transformed human B cells (HLA phenotype A11,A24,B27,B44,Bw4,Cw2). Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells. The antibody is cytotoxic for all HLA A11 positive cells and HLA A24 cells at an appropriate titer. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 5 X 10 exp5 cells/ml. |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG3 |
| Name of Depositor： | F Grumet |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Foung SK, et al. A monoclonal antibody against HLA-A11 and A-24. Hum. Immunol. 15: 316-319, 1986. PubMed: 2420768 Grumet FC, et al. Monoclonal antibody (B27M2) subdividing HLA-B27. Hum. Immunol. 5: 61-72, 1982. PubMed: 6981637 |