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| 产品名称： | a Intermediate filament alpha intermediate filament, anti intermediate filament |
|---|---|
| 商品货号： | TS212752 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with all classes of intermediate filaments including glial filaments in astrocytes. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against intermediate filaments |
| Cellular Products： | immunoglobulin; monoclonal antibody; against intermediate filaments |
| Comments： | Animals were immunized with human glial fibrillary acidic protein. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with all classes of intermediate filaments including glial filaments in astrocytes. Neurofilaments in axons. Tonofilaments in epithelial PtK2 cells and intermediate filaments in fibroblasts. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype： | IgG1 |
| Name of Depositor： | R Pruss |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Pruss RM, et al. All classes of intermediate filaments share a common antigenic determinant defined by a monoclonal antibody. Cell 27: 419-428, 1981. PubMed: 6086105 |