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微信小章| 产品名称： | 9-B1 |
|---|---|
| 商品货号： | TS212760 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody binds to and inhibits the catalytic activity of rat Na,K dependent ATPase. The antibody has been used to affinity purify the enzyme, and in immunohistocytochemical localization of the enzyme in rat tissues. |
| Derivation： | Animals were immunized with purified rat Na,K dependent ATPase. Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against rat Na,K dependent ATPase |
| Cellular Products： | immunoglobulin; monoclonal antibody; against rat Na,K dependent ATPase |
| Comments： | Animals were immunized with purified rat Na,K dependent ATPase. Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells. The antibody binds to and inhibits the catalytic activity of rat Na,K dependent ATPase. The antibody appears to bind to the alpha subunit of the enzyme. The antibody has been used to affinity purify the enzyme, and in immunohistocytochemical localization of the enzyme in rat tissues. |
| Complete Growth Medium： | The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG1 |
| Name of Depositor： | HL Leffert |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Schenk DB, et al. Use of a monoclonal antibody to quantify (Na+, K+) ATPase activity and sites in normal and regenerating rat liver. J. Biol. Chem. 259: 14941-14951, 1984. PubMed: 6094583 Sutherland E, et al. Biochemical localization of hepatic surface-membrane Na+, K+-ATPase activity depends on membrane lipid fluidity. Proc. Natl. Acad. Sci. USA 85: 8673-8677, 1988. PubMed: 2847169 Schenk DB, Leffert HL. Monoclonal antibodies to rat Na+, K+-ATPase block enzymatic activity. Proc. Natl. Acad. Sci. USA 80: 5281-5285, 1983. PubMed: 6310568 Leffert HL, et al. Hepatic (Na+, K+)-ATPase: a current view of its structure, function and localization in rat liver as revealed by studies with monoclonal antibodies. Hepatology 5: 501-507, 1985. PubMed: 2987099 Hubert JJ, et al. Rat hepatic (Na+, K+)-ATPase: alpha-Subunit isolation by immunoaffinity chromatography and structural analysis by peptide mapping. Biochemistry 25: 4156-4163, 1986. PubMed: 3017414 |