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| 产品名称： | 9AE10 |
|---|---|
| 商品货号： | TS212761 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with an antigen present on virtually all rabbit T cells and thymocytes. It reacts with 30% of rabbit bone marrow cells, and with a majority of cells in the brain. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against rabbit T cells (T lymphocytes) and thymocytes (cytotoxic) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against rabbit T cells (T lymphocytes) and thymocytes (cytotoxic) |
| Tumorigenic： | Yes |
| Effects： | Yes, in BALB/c mice |
| Comments： | Animals were immunized with rabbit spleen cells. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with an antigen present on virtually all rabbit T cells and thymocytes. It reacts with 30% of rabbit bone marrow cells, and with a majority of cells in the brain. The antigen is a glycoprotein with an apparent molecular weight of 25000 daltons and may be a rabbit analogue of the Thy-1 antigen. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | RPMI 1640 medium, 85%; horse serum, 10%; fetal bovine serum, 5%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgM |
| Name of Depositor： | KL Knight |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | McNicholas JM, et al. Monoclonal antibodies to rabbit lymphoid cells: preparation and characterization of a T-cell-specific antibody. Mol. Immunol. 18: 815-822, 1981. PubMed: 7040945 McNicholas JM, et al. Functional studies of rabbit T lymphocytes. Immunology 43: 635-641, 1981. PubMed: 6456220 |