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| 产品名称： | 9BG5 |
|---|---|
| 商品货号： | TS212762 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody neutralizes reovirus type 3 and inhibits the hemagglutinating activity of the virus. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Animals were immunized with purified reovirus type 3 virions. Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against reovirus type 3 (sigma 1 hemagglutinin) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against reovirus type 3 (sigma 1 hemagglutinin) |
| Comments： | Animals were immunized with purified reovirus type 3 virions. Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. The antibody neutralizes reovirus type 3 and inhibits the hemagglutinating activity of the virus. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG2a |
| Name of Depositor： | BN Fields |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Burstin SJ, et al. Evidence for functional domains on the reovirus type 3 hemagglutinin. Virology 117: 146-155, 1982. PubMed: 6175082 |