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| 产品名称： | 8A3B.6 |
|---|---|
| 商品货号： | TS212769 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody is specific for the structural protein VP7. |
| Derivation： | Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against VP7 of bluetongue virus |
| Cellular Products： | immunoglobulin; monoclonal antibody; against VP7 of bluetongue virus |
| Tumorigenic： | Yes |
| Effects： | Yes, in BALB/c mice |
| Comments： | Animals were immunized with bluetongue virus (serotype 17). Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody is specific for the structural protein VP7. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose, 2 mM L-glutamine and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 6 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 4 X 10 exp5 cells/ml. |
| Isotype： | IgG2a |
| Name of Depositor： | JA House |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Appleton JA, Letchworth GJ. Monoclonal antibody analysis of serotype-restricted and unrestricted bluetongue viral antigenic determinants. Virology 124: 286-299, 1983. PubMed: 6186076 House C, et al. Detection of bluetongue group-specific antibody by competitive ELISA. J. Vet. Diagn. Invest. 2: 137-139, 1990. PubMed: 1965579 |