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微信小章| 产品名称： | 8E5 derivative of CEM |
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| 商品货号： | TS212770 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | lymphoblast human immunodeficiency virus (HIV) positive |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2 xa0Cells contain Retrovirus
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | acute lymphoblastic leukemia |
| Age： | 4 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Clinical Data： | female Caucasian 4 years |
| Antigen Expression： | CD4 +; CD5 + |
| Receptor Expression： | transferrin |
| Genes Expressed： | most human immunodeficiency virus (HIV, HTLV III, LAV) structural proteins |
| Cellular Products： | most human immunodeficiency virus (HIV, HTLV III, LAV) structural proteins |
| Comments： | The cells contain a single defective proviral genome of HTLV III (HIV, LAV). All of the major structural protein are constitutively expressed with the exception of the p64 and p34 proteins. No infectious virus is produced; however, co-cultivation with Leu-3 + cells leads to syncytia formation. The cells have been cured of a mycoplasma contamination. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105xa0viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 106xa0cells/mL.
Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days. |
| Cryopreservation： | Complete growth medium 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | The United States of America |
| U.S. Patent Number： | |
| References： | Powell DM, et al. Cell line producing AIDS viral antigens without producing infectious virus particles. US Patent 4,752,565 dated Jun 21 1988 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |