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| 产品名称： | 89MS30 |
|---|---|
| 商品货号： | TS212774 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma:lymphoblast B lymphocyte; somatic cell hybri |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension, with some loosely adherent cells |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | trachoma |
| Strain： | Strain: BALB/cByJ (B cell); BALB/c (myeloma) |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against a chlamydia genus antigen |
| Cellular Products： | immunoglobulin; monoclonal antibody; against a chlamydia genus antigen |
| Comments： | Animals were immunized with C. trachomatis elementary bodies. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Attached cells may be subcultured by scraping. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG2b lambda , IgG2b; kappa light chain |
| Name of Depositor： | University of Massachusetts |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| U.S. Patent Number： | |
| References： | MacDonald B, et al. Oral vaccine comprising anti-idiotypic antibody to chlamydia glycolipid exoantigen and process. US Patent 5,656,271 dated Aug 12 1997 |