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微信小章| 产品名称： | 90196B |
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| 商品货号： | TS212776 |
| Organism： | Homo sapiens, human |
| Tissue： | spleen |
| Product Format： | frozen |
| Morphology： | monocyte/macrophage |
| Culture Properties： | suspension |
| Biosafety Level： | 2 xa0Cells contain Epstein-Barr DNA viral sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | The 90196B cell line was established from cryopreserved human spleen cells. |
| Comments： | The cells tend to grow in floating clusters.
Single cells may become adherent. |
| Complete Growth Medium： | Iscoves modified Dulbeccos medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
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| Subculturing： | The cells tend to grow in floating clusters. Single cells may become adherent. Cultures can be maintained by addition or replacement of fresh medium. Maintain the cell concentration between 2 x 105 and 1x106cells/mL. Gently pipette to break large clusters into smaller clusters. Medium Renewal: Twice weekly |
| Cryopreservation： | Culture medium,85%; DMSO,10%; extra FBS,10%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
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| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | E.I. du Pont de Nemours & Co. |
| Deposited As： | Homo sapiens |
| U.S. Patent Number： | |
| References： | Collins GW, Largen MT. Continuous mammalian cell lines having monocyte/macrophage characteristics and their establishment in vitro. US Patent 5,447,861 dated Sep 5 1995 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |