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微信小章| 产品名称： | 7F2 |
|---|---|
| 商品货号： | TS212783 |
| Organism： | Mus musculus, mouse |
| Tissue： | bone marrow |
| Cell Type： | osteoblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 5 to 6 weeks |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images： |  |
| Derivation： | 7F2 is a spontaneously immortalized cell line isolated from p53-/- mouse bone marrow taken from the femur and subsequently cloned. (Moore EE. Preparation of immortalized cells. US Patent 5,830,682 dated Nov 3 1998) |
| Clinical Data： | male |
| Genes Expressed： | alkaline phosphatase |
| Comments： | The cells appeared to correspond to mature osteoblasts because they express alkaline phosphatase, secrete type I collagen, show a significant cyclic adenosine monophosphate response to parathyroid hormone, secrete osteocalcin, and mineralize extensively. However, in contrast to mature osteoblasts, this clone can be induced to undergo massive adipocyte differentiation, and this differentiation is accompanied by the complete loss of expression of all osteoblastic markers except alkaline phosphatase. These observations indicate that some cells that have acquired all of the characteristics of mature osteoblasts can be diverted to the adipocyte pathway.xa0Ref |
| Complete Growth Medium： | Alpha minimum essential medium with 2 mM l-glutamine and 1 mM sodium pyruvate without ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%
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| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.
Subculture before the cells become confluent. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | ZymoGenetics, Inc. |
| Deposited As： | mouse |
| U.S. Patent Number： | |
| References： | Moore EE. Preparation of immortalized cells. US Patent 5,830,682 dated Nov 3 1998 Thompson DL, et al. The derivation and characterization of stromal cell lines from the bone marrow of p53-/- mice: new insights into osteoblast and adipocyte differentiation. J. Bone Miner. Res. 13: 195-204, 1998. PubMed: 9495512 |
Thompson DL, et al. The derivation and characterization of stromal cell lines from the bone marrow of p53-/- mice: new insights into osteoblast and adipocyte differentiation. J. Bone Miner. Res. 13: 195-204, 1998. PubMed: 9495512