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| 产品名称： | 74-22-15 |
|---|---|
| 商品货号： | TS212800 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts precipitates a 230000 dalton antigen from the surface of swine macrophages. A switch variant that produces IgG2b is also available (74-22-15A, TS212800.1). Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against pig macrophage and granulocyte; cytotoxic |
| Cellular Products： | immunoglobulin; monoclonal antibody; against pig macrophage and granulocyte; cytotoxic |
| Comments： | Animals were immunized with thymocytes from dd miniature swine. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts precipitates a 230000 dalton antigen from the surface of swine macrophages. A switch variant that produces IgG2b is also available (74-22-15A, TS212800.1). Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Culture Medium: Modified Dulbeccos medium (see below), 90%; fetal bovine serum, 10%. DME w/HEPES (10mM) 100 mL L-Glutamine (100x) 1 mL Solution I 1 mL Non-essential amino acids (100x) 1 mL NCTC 135 10 mL Fetal Bovine Serum 12 mL Solution I (100x) 1. 1320 mg oxalacetic acid (100 mM, MW 132). 2. 80 mg crystalline bovine insulin (20 units/mL, 25 units/mg). Add 1 and 2, stir at 37°C. Add Na pyruvate 550 mg (50 mM, FW 110). Bring up to 100 mL with distilled water. Stir at 37°C until solution dissolves. Filter, aliquot and store frozen. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG1; kappa light chain |
| Name of Depositor： | JR Lunney |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Pescovitz MD, et al. Preparation and characterization of monoclonal antibodies reactive with porcine PBL. J. Immunol. 133: 368-375, 1984. PubMed: 6609988 Lunney JK, et al. Overview of the first international workshop to define swine leukocyte cluster of differentiation (CD) antigens. Vet. Immunol. Immunopathol. 43: 193-206, 1994. PubMed: 7856053 |