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微信小章| 产品名称： | 72A1 |
|---|---|
| 商品货号： | TS212802 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with the 350000/220000 dalton viral envelope glycoprotein. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with disrupted fixed Epstein-Barr virus particles.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
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| Genes Expressed： | immunoglobulin; monoclonal antibody; against Epstein-Barr virus (EBV) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against Epstein-Barr virus (EBV) |
| Comments： | The antibody reacts with the 350000/220000 dalton viral envelope glycoprotein.
Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105xa0viable cells/mL. Maintain cultures at a cell concentration between 1 x 105xa0and 1 x 106xa0cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Complete growth medium supplemented with 5% (v/v) DMSO.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
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| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | mouse IgG1 |
| Name of Depositor： | GJ Hoffman |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Hoffman GJ, et al. Monoclonal antibody against a 250,000-dalton glycoprotein of Epstein-Barr virus identifies a membrane antigen and a neutralizing antigen. Proc. Natl. Acad. Sci. USA 77: 2979-2983, 1980. PubMed: 6248876 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |