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| 产品名称： | 7-34-1 |
|---|---|
| 商品货号： | TS212803 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody react with the MHC class I antigen of swine, and is probably heavy chain specific. |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against swine leucocyte antigen (SLA) a, c and d haplotypes (cytotoxic) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against swine leucocyte antigen (SLA) a, c and d haplotypes (cytotoxic) |
| Comments： | Animals were immunized with peripheral blood mononuclear cells (PMNC) from SLA c haplotype miniature swine. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody react with the MHC class I antigen of swine, and is probably heavy chain specific. It does not cross-react with PMNC from humans, sheep, goats or mice, but does cross-react with cells from commercial varieties of pigs. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Medium Renewal: 2 to 3 times per week Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG2a |
| Name of Depositor： | CM Warner |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Lie WR, et al. Preparation and characterization of murine monoclonal antibodies to swine lymphocyte antigens. Immunology 64: 599-605, 1988. PubMed: 2459049 |