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| 产品名称： | 60bca |
|---|---|
| 商品货号： | TS212808 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with human CD14, inhibits the binding of LPS - LPS binding protein complexes to CD14 on human phagocytes and prevents the release of tumor necrosis factor alpha. |
| Derivation： | Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human CD14 |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human CD14 |
| Comments： | Animals were immunized with cultured human peripheral blood monocytes. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with human CD14, inhibits the binding of LPS - LPS binding protein complexes to CD14 on human phagocytes and prevents the release of tumor necrosis factor alpha. |
| Complete Growth Medium： | The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG1; kappa light chain |
| Name of Depositor： | RF Todd |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Ashmun RA, et al. Expression of the human monocyte membrane antigen gp55 by murine fibroblasts after DNA-mediated gene transfer. Blood 69: 886-892, 1987. PubMed: 3545322 Wright SD, et al. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science 249: 1431-1433, 1990. PubMed: 1698311 |